The 6-hydroxydopamine Rat Model of Parkinson's Disease
| Autor: | Danilo Leandro Ribeiro, Keila Bariotto Dos Santos, Fernando E. Padovan-Neto, Rayanne Poletti Guimarães, Lívea Dornela Godoy, Mirella Rosine Corrêa |
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| Rok vydání: | 2021 |
| Předmět: |
animal structures
Parkinson's disease Dopamine General Chemical Engineering Substantia nigra General Biochemistry Genetics and Molecular Biology medicine Animals Neurotoxin Oxidopamine Medial forebrain bundle Hydroxydopamine General Immunology and Microbiology business.industry Pars compacta Dopaminergic Neurons General Neuroscience Neurodegeneration Dopaminergic Medial Forebrain Bundle Parkinson Disease/etiology Parkinson Disease medicine.disease Rats Substantia Nigra Disease Models Animal nervous system Dopaminergic Neurons/pathology business Neuroscience Substantia Nigra/pathology |
| Zdroj: | Guimarães, R P, Ribeiro, D L, Dos Santos, K B, Godoy, L D, Corrêa, M R & Padovan-Neto, F E 2021, ' The 6-hydroxydopamine Rat Model of Parkinson's Disease ', Journal of visualized experiments : JoVE, no. 176 . https://doi.org/10.3791/62923 |
| ISSN: | 1940-087X |
| DOI: | 10.3791/62923-v |
| Popis: | Motor symptoms of Parkinson's disease (PD)-bradykinesia, akinesia, and tremor at rest-are consequences of the neurodegeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc) and dopaminergic striatal deficit. Animal models have been widely used to simulate human pathology in the laboratory. Rodents are the most used animal models for PD due to their ease of handling and maintenance. Moreover, the anatomy and molecular, cellular, and pharmacological mechanisms of PD are similar in rodents and humans. The infusion of the neurotoxin, 6-hydroxydopamine (6-OHDA), into a medial forebrain bundle (MFB) of rats reproduces the severe destruction of dopaminergic neurons and simulates PD symptoms. This protocol demonstrates how to perform the unilateral microinjection of 6-OHDA in the MFB in a rat model of PD and shows the motor deficits induced by 6-OHDA and predicted dopaminergic lesions through the stepping test. The 6-OHDA causes significant impairment in the number of steps performed with the contralateral forelimb. |
| Databáze: | OpenAIRE |
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