Label free optical transmission tomography for biosystems: intracellular structures and dynamics
| Autor: | Viacheslav Mazlin, Olivier Thouvenin, Samer Alhaddad, Martine Boccara, Claude Boccara |
|---|---|
| Přispěvatelé: | Institut Langevin - Ondes et Images (UMR7587) (IL), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), European Project: 957546,Odysseus, European Project: 754387 |
| Rok vydání: | 2022 |
| Předmět: |
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics]
Biological Physics (physics.bio-ph) FOS: Physical sciences Physics - Biological Physics Medical Physics (physics.med-ph) Physics - Medical Physics Article Atomic and Molecular Physics and Optics Physics - Optics Optics (physics.optics) Biotechnology |
| Zdroj: | Biomedical optics express Biomedical optics express, 2022, 13 (8), pp.4190. ⟨10.1364/BOE.453586⟩ Biomed Opt Express |
| ISSN: | 2156-7085 |
| Popis: | International audience; There is an increasing need for label free methods that could reveal intracellular structures and dynamics. In this context, we develop a new optical tomography method working in transmission - full-field optical transmission tomography (FF-OTT). The method can measure the forward scattering signals and reveals the time-dependent metabolic signals in living cells. FF-OTT is a common path interferometer taking advantage of the Gouy phase shift - a π phase shift that the light wave experiences around the focus. By modulating the position of the focus one can alter the phase of the scattered light. Demodulation of images with different phases rejects the background and enhances the light from the depth-of-field, thus producing an optical section. We test FF-OTT by imaging single-cell diatoms and ex vivo biological samples. In fresh samples, we show that the intracellular motions create visible intensity fluctuations in FF-OTT so that the method is able to reveal a metabolic dynamic contrast. FF-OTT was found to be an efficient label free technique that can be readily implemented thanks to a robust common-path speckle-free interferometer design using an incoherent light source. |
| Databáze: | OpenAIRE |
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