Caspase-3 Cleaves the Expanded Androgen Receptor Protein of Spinal and Bulbar Muscular Atrophy in a Polyglutamine Repeat Length-Dependent Manner
| Autor: | Shigeru Miwa, Diane E. Merry, Li Mei, Manabu Doyu, Yasushi Kobayashi, Akito Kume, Gen Sobue |
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| Rok vydání: | 1998 |
| Předmět: |
Bulbar Palsy
Progressive Biophysics Caspase 3 Biology Biochemistry Muscular Atrophy Spinal Gene product Pathogenesis medicine Protein biosynthesis Humans Motor Neuron Disease Receptor Molecular Biology Genetics Enzyme Precursors Cell Biology Polyglutamine tract medicine.disease Recombinant Proteins Cell biology Androgen receptor Kinetics Spinal and bulbar muscular atrophy Receptors Androgen Caspases Protein Biosynthesis Peptides |
| Zdroj: | Biochemical and Biophysical Research Communications. 252:145-150 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.9624 |
| Popis: | Spinal and bulbar muscular atrophy (SBMA) is one of a group of human inherited neurodegenerative diseases caused by polyglutamine expansion. There is increasing evidence that generation of truncated proteins containing an expanded polyglutamine tract may be an important step in the pathogenesis of these disorders. We have previously demonstrated that the SBMA gene product, the androgen receptor (AR) protein, is toxic when truncated. We now report that in vitro translated full-length AR proteins containing different sized polyglutamine repeats (24, 65 and 97 repeats, respectively) are specifically cleaved by recombinant caspase-3, liberating a polyglutamine containing fragment, and that the susceptibility to cleavage is polyglutamine repeat length-dependent. These findings suggest that AR protein is one of the "death substrates" cleaved by caspase-3 and that caspase-3 might be involved in the pathogenesis of SBMA. |
| Databáze: | OpenAIRE |
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