Plexin-B2 regulates the proliferation and migration of neuroblasts in the postnatal and adult subventricular zone
| Autor: | Camille Boutin, Bhaskar Saha, Alain Chédotal, Athéna R Ypsilanti, Harold Cremer |
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| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Hepatocyte Growth Factor
Male Cerebellum Rostral migratory stream Antimetabolites animal diseases MESH: Neurons MESH: Mice Knockout MESH: Prosencephalon Mice 0302 clinical medicine Cell Movement MESH: Animals MESH: Nerve Tissue Proteins MESH: Cell Movement In Situ Hybridization MESH: Bromodeoxyuridine Mice Knockout Neurons 0303 health sciences biology Hepatocyte Growth Factor General Neuroscience Articles Immunohistochemistry Olfactory Bulb medicine.anatomical_structure Electroporation MESH: Antimetabolites embryonic structures Female MESH: Glial Cell Line-Derived Neurotrophic Factor MESH: Olfactory Bulb MESH: Mutation animal structures Neurogenesis Subventricular zone Nerve Tissue Proteins [SDV.BC]Life Sciences [q-bio]/Cellular Biology 03 medical and health sciences MESH: In Situ Hybridization Prosencephalon Semaphorin Neuroblast medicine Animals MESH: Electroporation Brain Tissue Transplantation Glial Cell Line-Derived Neurotrophic Factor MESH: Mice 030304 developmental biology Plexin MESH: Immunohistochemistry MESH: Male Olfactory bulb MESH: Neurogenesis nervous system Bromodeoxyuridine Forebrain Mutation biology.protein MESH: Brain Tissue Transplantation Neuroscience MESH: Female 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience, 2012, 32 (47), pp.16892-905. ⟨10.1523/JNEUROSCI.0344-12.2012⟩ Journal of Neuroscience, Society for Neuroscience, 2012, 32 (47), pp.16892-905. ⟨10.1523/JNEUROSCI.0344-12.2012⟩ The Journal of Neuroscience, Vol. 32, no.47, p. 16892-905 (2012) |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/JNEUROSCI.0344-12.2012⟩ |
| Popis: | In the postnatal forebrain, the subventricular zone (SVZ) contains a pool of undifferentiated cells, which proliferate and migrate along the rostral migratory stream (RMS) to the olfactory bulb and differentiate into granule cells and periglomerular cells. Plexin-B2 is a semaphorin receptor previously known to act on neuronal proliferation in the embryonic brain and neuronal migration in the cerebellum. We show here that, in the postnatal and adult CNS, Plexin-B2 is expressed in the subventricular zone lining the telencephalic ventricles and in the rostral migratory stream. We analyzedPlxnb2−/−mice and found that there is a marked reduction in the proliferation of SVZ cells in the mutant. Plexin-B2 expression is downregulated in the olfactory bulb as interneurons initiate radial migration. BrdU labeling and GFP electroporation into postnatal SVZ, in addition to time-lapse videomicroscopy, revealed that neuroblasts deficient for Plexin-B2 migrate faster than control ones and leave the RMS more rapidly. Overall, these results show that Plexin-B2 plays a role in postnatal neurogenesis and in the migration of SVZ-derived neuroblasts. |
| Databáze: | OpenAIRE |
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