The role of PA-X C-terminal 20 residues of classical swine influenza virus in its replication and pathogenicity
| Autor: | Xiao-Qian Gong, Qi Wang, Feng Wen, Xiu-Hui Wang, Hai Yu, Guangzhi Tong, Shuai-Yong Wang, Wu Tong, Lingxue Yu, Juan Wang, Ning Kong, Xiao-Min Liu, Tongling Shan, Yi-Feng Zhang, Hao Zheng, Yan-Jun Zhou, Bao-Yang Ruan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Swine
viruses Virulence Viral Nonstructural Proteins Biology Kidney Virus Replication Microbiology Virus Cell Line Madin Darby Canine Kidney Cells Mice 03 medical and health sciences Dogs Influenza A Virus H1N1 Subtype Orthomyxoviridae Infections Animals Humans Gene 030304 developmental biology Swine Diseases chemistry.chemical_classification Mice Inbred BALB C 0303 health sciences General Veterinary 030306 microbiology General Medicine Fusion protein Virology Reverse genetics Amino acid Repressor Proteins Open reading frame HEK293 Cells Viral replication chemistry A549 Cells Female |
| Zdroj: | Veterinary Microbiology. 251:108916 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2020.108916 |
| Popis: | PA-X is a fusion protein encoded by a +1 frameshifted open reading frame (X-ORF) in PA gene. The X-ORF can be translated in full-length (61 amino acids, aa) or truncated (41 aa) form. However, the role of C-Terminal 20 aa of PA-X in virus function has not yet been fully elucidated. To this end, we constructed the contemporary influenza viruses with full and truncated PA-X by reverse genetics to compare their replication and pathogenicity. The full-length PA-X virus in MDCK and human A549 cells conferred 10- to 100-fold increase in viral replication, and more virulent and caused more severe inflammatory responses in mice relative to corresponding truncated PA-X virus, suggesting that the terminal 20 aa could play a role in enhancing viral replication and contribute to virulence. |
| Databáze: | OpenAIRE |
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