Biomarkers and assessment of vaccine responses
| Autor: | Wayne R Hogrefe |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Health
Toxicology and Mutagenesis T-Lymphocytes Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Vaccine antigen Major histocompatibility complex Biochemistry Cancer Vaccines Models Biological Epitopes Immune system Animals Humans B-Lymphocytes Vaccines biology ELISPOT Antibody titer Vaccine efficacy Flow Cytometry Virology Infectious disease (medical specialty) Immunoglobulin G Immunology biology.protein Antibody Biomarkers |
| Zdroj: | Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 10 |
| ISSN: | 1354-750X |
| Popis: | Vaccines for infectious diseases have in the past, and will into the future, relied on a variety of surrogate markers to monitor vaccine efficacy. The primary surrogate markers have been either the antibody titer to vaccine antigens or the measurement of antibody function such as anti-viral neutralizing activity. In recent years, the measurement of T-cell function in conjunction with or independent of antibody measurements have been used to assess vaccine efficacy. ELISPOT, flow cytometry and intra-cellular staining methods are used to determine the impact of vaccines on immune mediators such as interleukins, interferons, MHC expression and pro-inflammatory mediators. The relevant B-cell and T-cell surrogate markers for vaccine efficacy is dependent on the vaccine being used, so that no universal set of surrogate markers can be applied to all vaccines. The use of T-cell surrogate markers can be complicated by the lack of sensitivity to accurately measure intra-cellular mediators. Although typically this is not a problem for infectious disease vaccines, it is a major problem for cancer vaccines. |
| Databáze: | OpenAIRE |
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