Relationship between the neural dysgenesis and increased production of class I MHC H-2Kk mRNA and protein in neurons of murine trisomy 16 fetuses
| Autor: | Kenneth J. Mack, Edward T. Bersu, Steven Kornguth |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Biophysics Gestational Age Trisomy In situ hybridization Biology Biochemistry Translocation Genetic Mice Exon Dysgenesis Fetus Complementary DNA Gene expression medicine Animals RNA Messenger Molecular Biology Neurons Genetics Mice Inbred BALB C Messenger RNA Histocompatibility Antigens Class I H-2 Antigens Brain Chromosome Mapping Trisomy 16 Cell Biology medicine.disease Molecular biology Female DNA Probes |
| Zdroj: | Biochemical and Biophysical Research Communications. 179:102-107 |
| ISSN: | 0006-291X |
| Popis: | Neuronal cells from murine trisomy 16 fetuses have increased levels of class I MHC H-2Kk. To determine whether this increased level of H-2Kk protein product resulted from an increased synthesis of mRNA, a 33 base antisense cDNA probe complementary to a region in exon 2 of the H-2Kk sequence (nucleotide 392–424) was synthesized. This probe was used to examine, by in situ hybridization and immunohistochemistry, the neural distribution of H-2Kk mRNA and protein product. A marked elevation of the H-2Kk mRNA and protein were localized in mts16 neuronal populations that were susceptible to dysgenesis. The results implicate the expression of the H-2Kk in the neuropathology of mts16 and its human counterpart, Down syndrome. |
| Databáze: | OpenAIRE |
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