Interactions of dextransucrase purified from
| Autor: | Akhtar Mahmood, Shabeer Ahmad, Dimple Goyal, Sukesh Chander Sharma |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Polyphenols interaction Biophysics Dextransucrase activity QD415-436 macromolecular substances Biochemistry Dextransucrase Streptococcus mutans GA Gallic acid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Column chromatography SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis Tannic acid Gallic acid Biology (General) ORD Optical rotatory dispersion chemistry.chemical_classification biology TA Tannic acid CD Circular Dichroism biology.organism_classification 030104 developmental biology Enzyme chemistry PEG Polyethylene glycol Polyphenol 030220 oncology & carcinogenesis Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 26, Iss, Pp 100980-(2021) |
| ISSN: | 2405-5808 |
| Popis: | Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans. In the present study, the effect of polyphenols gallic acid and tannic acid was investigated on the dextransucrase activity. The enzyme was purified by ethanol precipitation followed by column chromatography by Sephadex G-200 gel chromatography, followed by PEG-400 treatment. The purified enzyme exhibited 52 fold enrichment with 17.5% yield and specific activity of 3.54 Units/mg protein. On SDS-PAGE enzyme protein gave a single band with a molecular weight of 160 kDa. Dextransucrase activity was inhibited 80–90% by 0.04 mM tannic acid (TA) or 0.4 mM gallic acid (GA) suggesting that tannic acid has 10- fold more inhibitory potential than gallic acid on the activity of dextransucrase. CD/ORD studies revealed modifications in the tertiary structure of enzyme protein in presence of tannic acid and gallic acid, which were further confirmed by fluorescence spectra of the protein in presence of tannic acid. These results suggest that inhibition of dextransucrase activity in S. mutans by polyphenols may have potential applications in the prevention and control of dental caries. Graphical abstract Image 1 Highlights i• Dextransuccrase an important enzyme of S. mutans is involved in the metabolism of sucrose. • Purified enzyme is inhibited (80-90%) by plant polyphenols. Observed inhibition is due to change in teritary structure. • S. mutans is an important cariogenic agent. • Plant polyphenols are good anticariogenic agents. |
| Databáze: | OpenAIRE |
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