Berberine modulates cisplatin sensitivity of human gastric cancer cells by upregulation of miR-203
| Autor: | Xue-Meng Xie, Wei-Jian Zhang, He-Yi You, Heng-Liang Zhu, Fei-Zhao Jiang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Berberine Apoptosis Pharmacology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Stomach Neoplasms Cell Line Tumor Medicine Humans Cell Proliferation Cisplatin Cell growth business.industry Cancer Cell Biology General Medicine medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology chemistry Drug Resistance Neoplasm 030220 oncology & carcinogenesis Cancer cell business miR-203 Apoptosis Regulatory Proteins Developmental Biology medicine.drug |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 52(8) |
| ISSN: | 1543-706X |
| Popis: | Chemotherapeutic resistance is the main reason of the failure in clinical treatment of gastric cancer. Berberine (BER) is the active compound of traditional Chinese medicine Huang Lian. The aim of this present study is to evaluate the effect of BER on cisplatin resistance in gastric cancer cells and to investigate its possible mechanism. Gastric cancer cell lines SGC-7901 and BGC-823 and their respective cisplatin-resistant variants SGC-7901/DDP and BGC-823/DDP were used in this study. We found that BER treatment significantly reversed cisplatin sensitivity and induced caspase-dependent apoptosis in SGC-7901/DDP and BGC-823/DDP cells; BER treatment induced miR-203 expression, and overexpression of miR-203 mimicked the cisplatin-sensitizing effect of BER. Importantly, we showed that miR-203 was able to target the 3'UTR of Bcl-w. Therefore, we conclude that BER treatment reduces cisplatin resistance of gastric cancer cells by modulating the miR-203/Bcl-w apoptotic axis. BER may be a novel agent to enhance chemotherapeutic responses in cisplatin-resistant gastric cancer patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink