Endothelin-1 Production during the Acute Chest Syndrome in Sickle Cell Disease
| Autor: | Harrison W. Farber, Tammy J. Conca, Stella Kourembanas, Marisa Tucci, Mark Brauer, Samuel I. Hammerman |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Lung Diseases Pulmonary and Respiratory Medicine Hemolytic anemia Anemia Sickle Cell Pulmonary Artery Peptide hormone Hematocrit Critical Care and Intensive Care Medicine Andrology medicine Animals Humans RNA Messenger Endothelin-1 medicine.diagnostic_test business.industry Radioimmunoassay medicine.disease Endothelin 1 Sickle cell anemia Acute chest syndrome Acute Disease Immunology RNA Cattle Female Endothelin receptor business |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 156:280-285 |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/ajrccm.156.1.9611085 |
| Popis: | To investigate the role of the endothelial-derived vasoactive mediator endothelin (ET-1) in the acute chest syndrome (ACS), we incubated bovine pulmonary artery endothelial cells (BPAEC) with red blood cells (equivalent to a hematocrit of 20%) and/or autologous plasma (1:10 dilution) from two patients during ACS and during routine clinic visits. Cellular RNA was analyzed for ET-1 transcripts by Northern analysis and ET-1 protein levels in BPAEC supernatants and in plasma measured by radioimmunoassay. ET-1 mRNA expression and protein levels increased in BPAEC exposed to plasma obtained during ACS; in contrast, exposure to plasma obtained during routine clinic visits did not alter BPAEC ET-1 mRNA expression or protein levels. Plasma ET-1 level was elevated during ACS, decreased during resolution, and remained slightly elevated during routine clinic visits. Plasma obtained from one patient 4 d prior to hospitalization for vasoocclusive crisis contained the highest ET-1 level and markedly increased BPAEC ET-1 mRNA expression and protein levels. In both patients, BPAEC ET-1 mRNA and protein expression in vitro and plasma ET-1 levels in vivo correlated with stage of disease and occurred in the absence of direct erythrocyte contact in vitro. These observations suggest that ET-1 production contributes to development of ACS. |
| Databáze: | OpenAIRE |
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