High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics
| Autor: | Armelle Marais, Nikolaos I. Katis, Varvara I. Maliogka, A.T. Katsiani, Thierry Candresse, Antonio Olmos, Leonidas Lotos, Sébastien Theil, Laurence Svanella-Dumas, Chantal Faure, Ana Belén Ruiz-García |
|---|---|
| Přispěvatelé: | Aristotle University of Thessaloniki, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research (IVIA) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Phytopathology and phytopharmacy Virologie lcsh:QR1-502 Genome Viral Biology lcsh:Microbiology Article Virus DNA sequencing 03 medical and health sciences Prunus food santé des plantes Virology diagnostics Closteroviridae pathologie végétale Phylogeny Plant Diseases virus phytopathogène Genetic diversity little cherry virus 1 Phylogenetic tree Genetic Variation High-Throughput Nucleotide Sequencing LChV1 intra-host variability high-throughput sequencing Sequence Analysis DNA fruit biology.organism_classification Phytopathologie et phytopharmacie Velarivirus metropolitan_transit.transit_stop [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy 030104 developmental biology Infectious Diseases Virus Diseases Evolutionary biology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology RNA Viral metropolitan_transit Cherry tree |
| Zdroj: | Viruses Viruses, MDPI, 2018, 10 (7), pp.385. ⟨10.3390/v10070385⟩ Viruses 7 (10), 385. (2018) Volume 10 Issue 7 Viruses, Vol 10, Iss 7, p 385 (2018) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v10070385⟩ |
| Popis: | UMR BFP - Equipe Virologie; International audience; Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|