Intracellular Kaposi's Sarcoma-Associated Herpesvirus Load Determines Early Loss of Immune Synapse Components
| Autor: | Wai K. Law, Laura Adang, Dean H. Kedes, Costin Tomescu |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Viral protein
Immunology Gene Dosage Down-Regulation medicine.disease_cause Major histocompatibility complex Microbiology Cell Line Immunological synapse Viral Proteins Immune system Downregulation and upregulation Virology MHC class I medicine Humans Gammaherpesvirinae Gene Silencing RNA Small Interfering Kaposi's sarcoma-associated herpesvirus Promoter Regions Genetic biology Histocompatibility Antigens Class I Viral Load Intercellular Adhesion Molecule-1 biology.organism_classification Platelet Endothelial Cell Adhesion Molecule-1 Gene Expression Regulation Insect Science Herpesvirus 8 Human biology.protein Pathogenesis and Immunity |
| Zdroj: | Journal of Virology. 81:5079-5090 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Lifelong infection is a hallmark of all herpesviruses, and their survival depends on countering host immune defenses. The human gammaherpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) encodes an array of proteins that contribute to immune evasion, including modulator of immune recognition 2 (MIR2), an E3 ubiquitin ligase. Exogenously expressed MIR2 downregulates the surface expression of several immune synapse proteins, including major histocompatibility complex (MHC) class 1, ICAM-1 (CD54), and PECAM (CD31). Although immunofluorescence assays detect this lytic gene in only 1 to 5% of cells within infected cultures, we have found that de novo infection of naive cells leads to the downregulation of these immune synapse components in a major proportion of the population. Investigating the possibility that low levels of MIR2 are responsible for this downregulation in the context of viral infection, we found that MIR2 transduction recapitulated the patterns of surface downregulation following de novo infection and that both MIR2 promoter activation, MIR2 expression level, and immune synapse component downregulation were proportional to the concentration of KSHV added to the culture. Additionally, MIR2-specific small interfering RNA reversed the downregulation effects. Finally, using a sensitive, high-throughput assay to detect levels of the virus in individual cells, we also observed that downregulation of MHC class I and ICAM-1 correlated with intracellular viral load. Together, these results suggest that the effects of MIR2 are gene dosage dependent and that low levels of this viral protein contribute to the widespread downregulation of immune-modulating cell surface proteins during the initial stages of KSHV infection. |
| Databáze: | OpenAIRE |
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