Expression of LY6D is induced at the surface of MCF10A cells by X-ray irradiation
| Autor: | Eva Maria Surmann, Maiko Kurosawa, Gene Kurosawa, Noboru Hashimoto, Anand D. Jeyasekharan, Koichi Furukawa, Ashok R. Venkitaraman, Yoshikazu Kurosawa, Vignesh Venkatraman |
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| Rok vydání: | 2012 |
| Předmět: |
Transcription
Genetic Cell Cycle Proteins Ataxia Telangiectasia Mutated Proteins Protein Serine-Threonine Kinases Proteomics GPI-Linked Proteins Real-Time Polymerase Chain Reaction Biochemistry Proto-Oncogene Proteins p21(ras) Antigen Cell Line Tumor Humans Irradiation RNA Small Interfering Molecular Biology DNA Primers biology Base Sequence Tumor Suppressor Proteins X-Rays Cell Biology Molecular biology DNA-Binding Proteins Checkpoint Kinase 2 Membrane protein Polyclonal antibodies biology.protein X ray irradiation Antibody Tumor Suppressor Protein p53 Cell Adhesion Molecules Mutagens |
| Zdroj: | The FEBS journal. 279(24) |
| ISSN: | 1742-4658 |
| Popis: | In order to identify membrane proteins whose expression is induced by X-ray irradiation, we developed an antibody (Ab)-directed strategy using a phage Ab library. X-Ray-irradiated cells were screened with a phage Ab library in the presence of a large excess of polyclonal Abs prepared against membrane proteins that are commonly present at the surface of both X-ray-irradiated and nonirradiated cells. After isolation of Ab that bound only to X-ray-irradiated cells, the antigen was identified using MS. Using this approach, we found that expression of LY6D is induced in MCF10A cells by X-ray irradiation. The induction of LY6D expression is triggered through a pathway regulated by ATM, CHK2 and p53. This method is a new Ab-directed proteomic strategy for analysis of membrane proteins, and is applicable to various biological phenomena in situations in which both target molecule-expressing cells and nonexpressing cells are available. |
| Databáze: | OpenAIRE |
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