The Arabidopsis L-Type Amino Acid Transporter 5 (LAT5/PUT5) Is Expressed in the Phloem and Alters Seed Nitrogen Content When Knocked Out
| Autor: | Allen G. Good, Rowshon A. Begam, Jayne D'Entremont |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antiporter Arabidopsis Plant Science 01 natural sciences Article nitrogen 03 medical and health sciences chemistry.chemical_compound PUT5 amino acid translocation Amino acid transporter Ecology Evolution Behavior and Systematics chemistry.chemical_classification Growth medium amino acids Ecology biology seed nitrogen Botany Wild type LAT5 biology.organism_classification L-type amino acid transporter Amino acid 030104 developmental biology LAT chemistry Biochemistry QK1-989 polyamine uptake transporter Phloem Leucine 010606 plant biology & botany |
| Zdroj: | Plants Plants, Vol 9, Iss 1519, p 1519 (2020) Volume 9 Issue 11 |
| ISSN: | 2223-7747 |
| Popis: | The Arabidopsis L-type Amino Acid Transporter-5 (LAT5 At3g19553) was recently studied for its role in developmental responses such as flowering and senescence, under an assumption that it is a polyamine uptake transporter (PUT5). The LATs in Arabidopsis have a wide range of substrates, including amino acids and polyamines. This report extensively studied the organ and tissue-specific expression of the LAT5/PUT5 and investigated its role in mediating amino acid transport. Organ-specific quantitative RT-PCR detected LAT5/PUT5 transcripts in all organs with a relatively higher abundance in the leaves. Tissue-specific expression analysis identified GUS activity in the phloem under the LAT5/PUT5 promoter. In silico analysis identified both amino acid transporter and antiporter domains conserved in the LAT5/PUT5 protein. The physiological role of the LAT5/PUT5 was studied through analyzing a mutant line, lat5-1, under various growth conditions. The mutant lat5-1 seedlings showed increased sensitivity to exogenous leucine in Murashige and Skoog growth medium. In soil, the lat5-1 showed reduced leaf growth and altered nitrogen content in the seeds. In planta radio-labelled leucine uptake studies showed increased accumulation of leucine in the lat5-1 plants compared to the wild type when treated in the dark prior to the isotopic feeding. These studies suggest that LAT5/PUT5 plays a role in mediating amino acid transport. |
| Databáze: | OpenAIRE |
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