Maintenance of neutralizing antibodies over ten months in convalescent SARS‐CoV‐2 afflicted patients
| Autor: | Norbert Nowotny, Christian Sturmbauer, Chantal Rodgarkia-Dara, Stephan Koblmüller, Carmen Schönegger, Alexander Tichy, Sarah Gietl, Tom Loney, Hanna Jury, Bianca Jansen, Martina Prelog, Gernot Walder, Giovanni Almanzar, Wilfried Posch, Sissy Therese Sonnleitner |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
040301 veterinary sciences
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Immunofluorescence Antibodies Viral Neutralization SARS‐CoV‐2 0403 veterinary science 03 medical and health sciences Immune system Immunity Medicine Animals neutralization test 030304 developmental biology 0303 health sciences General Veterinary General Immunology and Microbiology biology medicine.diagnostic_test business.industry SARS-CoV-2 COVID-19 04 agricultural and veterinary sciences General Medicine Original Articles persistence immunity Antibodies Neutralizing Immunity Humoral Titer Specific antibody Immunology biology.protein Original Article IgG antibodies Antibody business |
| Zdroj: | Transboundary and Emerging Diseases |
| ISSN: | 1865-1682 1865-1674 |
| Popis: | Knowledge of the level and duration of protective immunity against SARS‐CoV‐2 after primary infection is of crucial importance for preventive approaches. Currently, there is a lack of evidence on the persistence of specific antibodies. We investigated the generation and maintenance of neutralizing antibodies of convalescent SARS‐CoV‐2‐afflicted patients over a ten‐month period post‐primary infection using an immunofluorescence assay, a commercial chemiluminescent immunoassay and an in‐house enzyme‐linked neutralization assay. We present the successful application of an improved version of the plaque‐reduction neutralization assay which can be analysed optometrically to simplify data interpretation. Based on the results of the enzyme‐linked neutralization assay, neutralizing antibodies were maintained in 77.4% of convalescent individuals without relevant decay over ten months. Furthermore, a positive correlation between severity of infection and antibody titre was observed. In conclusion, SARS‐CoV‐2‐afflicted individuals have been proven to be able to develop and maintain neutralizing antibodies over a period of ten months after primary infection. Findings suggest long‐lasting presumably protective humoral immune responses after wild‐type infection. |
| Databáze: | OpenAIRE |
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