comparison of three yeast cytochrome C's purified from Saccharomyces cerevisiae, Kloeckera sp. and Candida krusei

Autor: Hiroshi Mizushima, Kazuo Okunuki, Tateo Yamanaka, Hamako Katano
Rok vydání: 1964
Předmět:
Zdroj: Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects. 85:11-17
ISSN: 0926-6569
DOI: 10.1016/0926-6569(64)90162-2
Popis: Three yeast cytochrome c 's purified from Saccharomyces cerevisiae, Kloeckera sp. and Candida krusei were compared. Their absorption spectra were almost the same, but their affinities for an Amberlite CG-50 column decreased in the order: Saccharomyces cytochrome c > Kloeckera cytochrome c > Candida cytochrome c . They showed the same reactivity with Pseudomonas cytochrome oxidase, whereas their reactivity with cow cytochrome a decreased in the order: Saccharomyces cytochrome c ⪖ Candida cytochrome c > Kloeckera cytochrome c . The latter order was observed in their electrophoretic mobilities also. Sacchaomyces and Candida cytochromes c 's were not precipitated from dilute solution by saturated ammonium sulphate solution whereas Kloeckera cytochrome c was appreciably precipitated.
Databáze: OpenAIRE