Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles
| Autor: | Shinji Komori, Koji Koyama, Akiko Hasegawa, Giannina Calongos |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Menotropins Cleavage Stage Ovum Embryonic Development Cell Count Fertilization in Vitro Biology Mice Follicle-stimulating hormone Follicle Oogenesis Human fertilization Ovarian Follicle Pregnancy Internal medicine Follicular phase medicine Animals Ovarian follicle Cell Proliferation Estradiol Obstetrics and Gynecology Antral follicle Oocyte Recombinant Proteins In vitro maturation Mice Inbred C57BL medicine.anatomical_structure Endocrinology Reproductive Medicine Mice Inbred DBA Female Follicle Stimulating Hormone hormones hormone substitutes and hormone antagonists |
| Zdroj: | Fertility and Sterility. 89:1482-1489 |
| ISSN: | 0015-0282 |
| Popis: | Objective To compare the effects of urinary and recombinant follicle-stimulating hormone (FSH) on follicular development, oocyte maturation, and fertilization. Design Prospective randomized animal study. Setting University-based research laboratory. Animal(s) Normal (C57BL/6xDBA2) F1 mice. Intervention(s) Collection of preantral follicles by mechanical dissection, in vitro growth (IVG) with urinary or recombinant FSH (100 mIU/mL), in vitro maturation (IVM), and fertilization. Main Outcome Measure(s) During IVG, follicle diameter and antral formation were evaluated. The number of cells per follicle was evaluated at the end of IVG. The 17β-estradiol measurements were performed from conditioned media. After IVM, mucification and oocyte maturation rates were estimated and mature oocytes were fertilized. Result(s) No differences were observed in the antral formation rate of either group. Antral follicles in the urinary FSH group, however, showed a higher diameter but a lower number of cells per follicle than those in the recombinant FSH group. The level of 17β-estradiol was also higher in the recombinant FSH group. The rate of mature oocytes developing into metaphase I or II was statistically significantly higher in the recombinant FSH group than in the urinary FSH group. Conclusion(s) Recombinant FSH provided better conditions than urinary FSH for the growth and development of mouse preantral follicles that could produce mature oocytes with fertilization capacity. |
| Databáze: | OpenAIRE |
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