Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing
| Autor: | Fintan Kelleher, Aoife Delaney, Jessica Kelch, Priscilla G. L. Baker, Emmanuel I. Iwuoha, Eithne Dempsey |
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| Rok vydání: | 2019 |
| Předmět: |
Hydrochloride
Transducers Biomedical Engineering Biophysics Enzyme-Linked Immunosorbent Assay 02 engineering and technology Biosensing Techniques Glassy carbon Electrochemistry 01 natural sciences Redox chemistry.chemical_compound Reaction rate constant Ferricyanides Semicarbazone Progesterone Immunoassay 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology Combinatorial chemistry Carbon 3. Good health 0104 chemical sciences Dielectric spectroscopy chemistry Dielectric Spectroscopy Gold 0210 nano-technology Antibodies Immobilized Oxidation-Reduction Derivative (chemistry) Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 150 |
| ISSN: | 1873-4235 |
| Popis: | Preparation and electrochemical interrogation of a novel redox active progesterone derivative progesterone thiosemicarbazone (PATC) is presented here together with an investigation into its suitability as conjugate in progesterone hormone immunosensing. PATC synthesis involved a condensation reaction between progesterone acetate and thiosemicarbazone hydrochloride. Voltammetric and pulse techniques confirmed the redox behaviour of the new compound with concentration and scan rate dependant irreversible behaviour evident at glassy carbon and gold transducers - ko (standard heterogeneous rate constant) was 2.56 � 10-3 cm2 /s (ν ¼ 100 mV/s in non-aqeuous media). Bioaffinity studies towards anti-progesterone antibodies involved a competitive ELISA format (optical) which confirmed recognition of the new progesterone derivative. Electrochemical impedance spectroscopy was employed as an interrogation technique in order to establish optimum binding and surface conditions for progesterone antigen-antibody interaction with the assistance of a redox probe (potassium hexacyanoferrate). |
| Databáze: | OpenAIRE |
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