Long-term effect of platelet lysate on primary fibroblasts highlighted with a proteomic approach
| Autor: | Laura Mazzucco, Maria Cavaletto, Valeria Balbo, Elia Ranzato, Mauro Patrone, Valentina Cipriani |
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| Rok vydání: | 2009 |
| Předmět: |
Blood Platelets
Proteomics Cell signaling Proteome Cell Survival MAP Kinase Signaling System p38 mitogen-activated protein kinases Biomedical Engineering Medicine (miscellaneous) Biology p38 Mitogen-Activated Protein Kinases Mass Spectrometry Biomaterials Western blot medicine Humans Platelet Viability assay Phosphorylation Cytoskeleton Skin Proteomic Profile Dose-Response Relationship Drug medicine.diagnostic_test Fibroblasts Biochemistry Gentian Violet Platelet lysate Wound healing Signal Transduction |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 3:531-538 |
| ISSN: | 1932-7005 1932-6254 |
| Popis: | The use of platelets and platelet derivatives has acquired clinical relevance as a means of accelerating wound healing. Platelet beneficial effect is attributed to the release of growth factors and other bioactive substances able to regulate cellular activities. The purpose of this study was to evaluate the biological effects of platelet lysate (PL) on human primary skin fibroblasts. We studied cell viability, MAPK signalling and proteomic profile of fibroblasts exposed to a platelet lysate (PL) obtained from blood sample. Crystal violet and neutral red uptake assays showed the dose–response effects of PL on cell viability and metabolism at 3 and 6 days of exposure. Western blot demonstrated a more sustained activation of p38 than of ERK1/2. A proteomic approach was applied to identify soluble cellular components in primary fibroblasts that are differentially expressed in response to PL exposure. Protein identification was performed by mass spectrometry. The data demonstrate that human fibroblasts respond to PL exposure by modifying a number of proteins, related principally to stress response, metabolism and the cytoskeleton. Copyright © 2009 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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