Comparison of two 2,3-diacyl trehalose antigens from Mycobacterium tuberculosis and Mycobacterium fortuitum for serology in tuberculosis patients
Autor: | M T Tórtola, M A Lanéelle, N Martín-Casabona |
---|---|
Jazyk: | angličtina |
Rok vydání: | 1996 |
Předmět: |
Microbiology (medical)
Serotype Tuberculosis Clinical Biochemistry Immunology Enzyme-Linked Immunosorbent Assay Immunoglobulin G Microbiology Serology Mycobacterium tuberculosis Antigen medicine Immunology and Allergy Humans Serotyping Antigens Bacterial biology Trehalose Nontuberculous Mycobacteria medicine.disease biology.organism_classification Antibodies Bacterial biology.protein Mycobacterium fortuitum Mycobacterium Research Article |
Popis: | Immunoglobulin G antibodies against two 2,3-diacyl trehalose (DAT) antigens from Mycobacterium tuberculosis (DATT) and Mycobacterium fortuitum (DATF) were studied by enzyme-linked immunosorbent assay of 356 serum samples. The sera were obtained from non-tuberculosis-infected individuals (282 serum samples) and tuberculosis patients (74 serum samples). Non-tuberculosis-infected individuals were healthy people (120 serum samples; positive purified-protein-derivative skin test, 60 patients; negative purified-protein-derivative skin test, 60 patients) patients with nontuberculosis lung disease (59 serum samples), contacts of sputum-smear-positive tuberculosis patients (57 serum samples), and human immunodeficiency virus-infected patients with nontuberculosis lung disease (46 serum samples). Of the 74 patients with tuberculosis, 14 were human immunodeficiency virus infected. The sensitivity of the method using DATT was 44.5%, and that with DATF was 48.6%. The specificities with both antigens were 99.1%. There were no significant differences between the mean values for both antigens (P = 0.2815). We therefore concluded that both antigens were interchangeable. As M. fortuitum, a fast-growing mycobacterium, could be a good source of antigen DAT, these results deserve consideration in the serology of tuberculosis. |
Databáze: | OpenAIRE |
Externí odkaz: |