The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
| Autor: | Carmen Milena Rodrigues Siqueira Carvalho, Isadora Mello Vilarinho Soares, Dayseanny de Oliveira Bezerra, Yulla Klinger de Carvalho Leite, Larissa Cordeiro Cavalcante, Maria Acelina Martins de Carvalho, Gustavo Vicentis de Oliveira Fernandes |
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| Přispěvatelé: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Pathology Bone Regeneration Time Factors Acute Inflammatory Infiltrate Stem cells Hemostatics 030207 dermatology & venereal diseases 0302 clinical medicine Osteogenesis Medicine Osteopontin Cells Cultured biology Stem cell transplantation Flow Cytometry Immunohistochemistry Bone regeneration Treatment Outcome Original Article Collagen Stem cell medicine.medical_specialty Cell Survival Mesenchymal Stem Cell Transplantation Aloe vera 03 medical and health sciences Herbal medicines Animals Humans Tibia Aloe General Dentistry Dental Pulp Plant Extracts business.industry Mesenchymal stem cell Reproducibility of Results 030206 dentistry biology.organism_classification Rats lcsh:RK1-715 Transplantation Microscopy Fluorescence lcsh:Dentistry biology.protein business |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Journal of Applied Oral Science, Volume: 27, Article number: e20180103, Published: 11 APR 2019 Journal of Applied Oral Science Journal of Applied Oral Science v.27 2019 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP Journal of Applied Oral Science, Vol 27, Iss 0 (2019) |
| Popis: | Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn’s post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon® (p |
| Databáze: | OpenAIRE |
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