Imaging Lipid Metabolism at the Golgi Complex
| Autor: | Giovanni D'Angelo, Serena Capasso |
|---|---|
| Předmět: |
chemistry.chemical_classification
0303 health sciences Phosphatidylinositol 4-phosphate Chemistry Biomolecule Lipid metabolism Computational biology Golgi apparatus Subcellular localization Sphingolipid 03 medical and health sciences chemistry.chemical_compound symbols.namesake 0302 clinical medicine symbols Molecular imaging 030217 neurology & neurosurgery 030304 developmental biology Diacylglycerol kinase |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493991358 |
| Popis: | The development of fluorescence-based molecular imaging has revolutionized cell biology allowing the visualization of specific biomolecules at the microscopic and, more recently, at the nanoscopic scale while in their relevant biological contexts. Nonetheless, despite the imaging toolkit for biologists interested in exploring the subcellular localization and dynamics of proteins and nucleic acids has expanded exponentially in the last decades, the means to visualize and track lipids in cells did not develop to the same extent until recently. Here we described some basic fluorescence-based techniques that can be used in standard cell biology laboratories to visualize subcellular pools of specific lipids and to evaluate their regional metabolism. Specifically, here we focus on the imaging-based analysis of phosphoinositide and sphingolipid metabolism at the Golgi complex. |
| Databáze: | OpenAIRE |
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