Control of Cytochrome P 1 -450 Gene Expression by Dioxin
| Autor: | James P. Whitlock, Phillip B. C. Jones, Donna R. Galeazzi, Joan M. Fisher |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Polychlorinated Dibenzodioxins Transcription Genetic Cytochrome CYP1B1 DNA Recombinant Dioxins Transfection Cell Line Mice Liver Neoplasms Experimental Cytochrome P-450 Enzyme System Acetyltransferases Transcription (biology) Genes Regulator Gene expression Animals Cytochrome P450 family 1 member A1 Promoter Regions Genetic Gene Transcription factor Regulation of gene expression Multidisciplinary biology Molecular biology Gene Expression Regulation Biochemistry Enzyme Induction biology.protein Transcription Factors |
| Zdroj: | Science. 227:1499-1502 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.3856321 |
| Popis: | The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) may produce its effects by altering gene expression in susceptible cells. In mouse hepatoma cells, TCDD induces the transcription of the cytochrome P1-450 gene, whose product, aryl hydrocarbon hydroxylase, contributes both to the detoxification and to the metabolic activation of carcinogenic polycyclic aromatic hydrocarbons. A DNA fragment containing sequences flanking the 5' end of the cytochrome P1-450 gene was isolated and analyzed. This DNA fragment contains a cis-acting control element with at least three functional domains: a putative promoter, an inhibitory domain upstream from the promoter that blocks its function, and a TCDD-responsive domain still farther (1265 to 1535 base pairs) upstream of the promoter. These findings, together with results from earlier studies, imply that transcription of the cytochrome P1-450 gene is under both positive and negative control by at least two trans-acting regulatory factors. |
| Databáze: | OpenAIRE |
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