Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark

Autor: Iben Anne Rasmussen, Linda Harkness, Moustapha Kassem, Karin Erb
Rok vydání: 2010
Předmět:
Zdroj: Harkness, L, Rasmussen, I A, Erb, K & Kassem, M 2010, ' Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark ', In Vitro Cellular & Developmental Biology-Animal, vol. 46, no. 3-4, pp. 259-268 . https://doi.org/10.1007/s11626-010-9281-7
ISSN: 1543-706X
1071-2690
DOI: 10.1007/s11626-010-9281-7
Popis: Udgivelsesdato: 2010-Mar-3 The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7% developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank ( http://www.ukstemcellbank.org.uk/ ).
Databáze: OpenAIRE
Externí odkaz: