Reduced nucleotomy-induced intervertebral disc disruption through spontaneous spheroid formation by the Low Adhesive Scaffold Collagen (LASCol)

Autor: Yutaro Kanda, Yohei Kawakami, Kaoru Omae, Toru Takada, Saori Kunii, Toshiyuki Takemori, Koichi Morimoto, Yoshiki Takeoka, Yuji Kakiuchi, Kotaro Nishida, Kenichiro Kakutani, Ryu Tsujimoto, Naomasa Fukase, Ryosuke Kuroda, Shingo Miyazaki, Takashi Yurube, Masanori Fukushima
Rok vydání: 2019
Předmět:
Pathology
medicine.medical_specialty
medicine.medical_treatment
Biophysics
Bioengineering
02 engineering and technology
Degeneration (medical)
Intervertebral Disc Degeneration
Matrix (biology)
Atelocollagen (AC)
Low Adhesive scaffold Collagen (LASCol)
Biomaterials
03 medical and health sciences
Myelopathy
Discectomy
Adhesives
medicine
Animals
Rat tail nucleotomy model of disc disruption
Intervertebral Disc
030304 developmental biology
0303 health sciences
Chemistry
Cell migration
Intervertebral disc
021001 nanoscience & nanotechnology
medicine.disease
Spine
Nucleotomy
Rats
Intervertebral disc degeneration and herniation
medicine.anatomical_structure
Mechanics of Materials
Biodegradable scaffold for tissue-engineering regeneration
Ceramics and Composites
Collagen
Stem cell
0210 nano-technology
Intervertebral Disc Displacement
Zdroj: Biomaterials. 235
ISSN: 1878-5905
Popis: Back pain is a global health problem with a high morbidity and socioeconomic burden. Intervertebral disc herniation and degeneration are its primary cause, further associated with neurological radiculopathy, myelopathy, and paralysis. The current surgical treatment is principally discectomy, resulting in the loss of spinal movement and shock absorption. Therefore, the development of disc regenerative therapies is essential. Here we show reduced disc damage by a new collagen type I-based scaffold through actinidain hydrolysis-Low Adhesive Scaffold Collagen (LASCol)-with a high 3D spheroid-forming capability, water-solubility, and biodegradability and low antigenicity. In human disc nucleus pulposus and annulus fibrosus cells surgically obtained, time dependent spheroid formation with increased expression of phenotypic markers and matrix components was observed on LASCol but not atelocollagen (AC). In a rat tail nucleotomy model, LASCol-injected and AC-injected discs presented relatively similar radiographic and MRI damage control; however, LASCol, distinct from AC, decelerated histological disc disruption, showing collagen type I-comprising LASCol degradation, aggrecan-positive and collagen type II-positive endogenous cell migration, and M1-polarized and also M2-polarized macrophage infiltration. Reduced nucleotomy-induced disc disruption through spontaneous spheroid formation by LASCol warrants further investigations of whether it may be an effective treatment without stem cells and/or growth factors for intervertebral disc disease.
Databáze: OpenAIRE
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