Reduction of the argyrophilic nucleolar organizing region associated protein synthesis with age in buccal epithelial cells of healthy individuals
| Autor: | Recep Eröz, Nalan Imamoglu, Halil Demirtas, Burak Selvi |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Buccal epithelial cells Aging Pathology medicine.medical_specialty Adolescent Nucleolus rDNA Biology Antigen medicine Protein biosynthesis Gene family Humans Child Aged Aged 80 and over AgNORs Age Factors Mouth Mucosa Antigens Nuclear Ribosomal RNA Middle Aged NOR Molecular biology Cell nucleus medicine.anatomical_structure Child Preschool Female Bone marrow Geriatrics and Gerontology Nucleolus organizer region |
| Popis: | WOS: 000351531100012 PubMed: 25082567 Background Nucleolus organizer regions (NORs) consist of the rRNA coding gene family (rDNA) in the cell nucleus. The argyrophilic proteins are selectively stained with silver nitrate and bind these regions. It was reported that NOR (rDNA) activity decreases in human lymphocytes, fibroblasts and bone marrow with age. However, to our knowledge there have not been any studies related to the NORs in oral epithelial cells of healthy individuals. Aim Our aim is to detect any correlation between age and Total AgNOR area/Total nucleus area (TAA/TNA) values in buccal epithelial cells of healthy individuals. Methods Oral epithelial cells from 50 healthy individuals (age range of 2-80 years old) were spread onto a clean glass slide, air dried and fixed. Then the AgNOR staining protocol was performed on these cells. TAA/TNA ratio and AgNOR dots were calculated using software. From each person 50 oral epithelial cells were evaluated. Results Statistically significant correlations were found between mean TAA/TNA values and age (Rsq = 0.534, p < 0.001 for linear and Rsq = 0.728, p < 0.0001 for polynominal regression), and between AgNOR number and age (Rsq = 0.621, p < 0.001 for linear and Rsq = 0.693, p < 0.0001 for polynominal regression). Conclusion There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age. Erciyes University Research FundErciyes University [TSY-08-559]; Medical Research Council of Erciyes University (ERUBAP)Erciyes University [TSY-08-559] Erciyes University Research Fund has supported this research; contract Grant Number: TSY-08-559. This study is the essential part of the MS thesis of Burak Selvi. The Medical Research Council of Erciyes University (ERUBAP, Project No. TSY-08-559) supported this work. |
| Databáze: | OpenAIRE |
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