Detection of HIV-1 RNA in heparinized plasma of HIV-1 seropositive individuals

Autor:	Takashi Kurimura, Osamu Yamada, Shinji Morita, Hideki Imai, Seishi Suehiro
Rok vydání:	1992
Předmět:	chemistry.chemical_classification Heparinase Heparin RNA Biology Virology Molecular biology HIV Reverse Transcriptase Reverse transcriptase Virus Heparin lyase Enzyme chemistry HIV Seropositivity Blood plasma HIV-1 medicine Humans RNA Viral Reverse Transcriptase Inhibitors medicine.drug
Zdroj:	Journal of Virological Methods. 36:181-184
ISSN:	0166-0934
DOI:	10.1016/0166-0934(92)90149-8
Popis:	The interference of reverse transcription by heparin was removed by heparinase. When the HIV-1 RNA in the presence of heparin was detected by a combination of reverse transcription and the polymerase chain reaction (PCR), heparinase treatment followed by removal of Ca2+ before the reverse transcription step permitted the efficient detection of HIV-1 RNA. Prior treatment with heparinase revealed HIV-1 RNA in 68% (13/19) of heparinized plasma samples from HIV-1 carriers, whereas only 26% (5/19) of the same specimens were positive without the heparinase step. Heparinase removed the inhibition of reverse transcription by heparin and is highly recommended when detecting low levels of viral RNA in heparinized plasma.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4762090d984c54880400d91d5bc67ba https://doi.org/10.1016/0166-0934(92)90149-8 Zobrazit plný text záznamu