Hormone-balancing and protective effect of combined extract of Sauropus androgynus and Elephantopus scaber against Escherichia coli-induced renal and hepatic necrosis in pregnant mice
| Autor: | Nurhayati, Muhaimin Rifa'i, Yuyun Ika Christina, Meyla Restia Diana, Eka Noviya Fuzianingsih, Widodo, Muhammad Sasmito Djati, Faradisa Noviani Ridwan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0211 other engineering and technologies
02 engineering and technology Pregnant medicine.disease_cause Andrology 03 medical and health sciences 0302 clinical medicine Blood serum Sauropus androgynous 021105 building & construction Drug Discovery medicine Miscellaneous systems and treatments Escherichia coli Kidney Pregnancy Hormonal biology business.industry Elephantopus scaber RZ409.7-999 Hepatic necrosis E. coli biology.organism_classification medicine.disease 030205 complementary & alternative medicine Staining medicine.anatomical_structure Complementary and alternative medicine Original Research Article (Experimental) business Hormone |
| Zdroj: | Journal of Ayurveda and Integrative Medicine Journal of Ayurveda and Integrative Medicine, Vol 12, Iss 2, Pp 245-253 (2021) |
| ISSN: | 0975-9476 |
| DOI: | 10.1016/j.jaim.2020.09.001 |
| Popis: | Background: Elephantopus scaber (ES) and Sauropus androgynous (SA) have been frequently reported to possess antibacterial activity through in vitro, but in vivo studies about the protective effect of combined ES and SA have acquired less attention. Objectives: To evaluate protective effect of combined ethanol extract of ES and SA on hormone imbalance and renal and hepatic necrosis formation in Escherichia coli-infected pregnant mice. Materials and methods: A total of 28 pregnant Balb/c mice were divided into seven groups (n = 4): control, E. coli-infected pregnant mice, infected pregnant mice received 200 mg/kg ES, infected pregnant mice received combined 150 mg/kg ES and 37.5 mg/kg SA (75:25), 100 mg/kg ES and 75 mg/kg SA (50:50), 50 mg/kg ES and 112.5 mg/kg SA (25:75), and only 150 mg/kg SA. Pregnant mice were orally treated with combined ES and SA on day 1–4th of pregnancy. On the 4th day, mice were infected with 107 CFU/mL of E. coli and continuously treated with ES and SA until the 16th day of pregnancy. After treatment, the kidney and liver were prepared for histological examination using H&E staining. The blood serum was collected in each stage of pregnancy and measured by ELISA assays. Results: Combined ES and SA gave an impact on altering the prolactin level. Combined ES and SA at ratio dose 75:25 was able to restore progesterone to normal levels (P |
| Databáze: | OpenAIRE |
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