How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis Thaliana
| Autor: | Juan Luis Santos, Mónica Pradillo, Pablo Parra-Nunez |
|---|---|
| Přispěvatelé: | Pradillo, Mónica, Heckmann, Stefan |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics biology medicine.diagnostic_test Botánica Chromosome biology.organism_classification 01 natural sciences Genética Chiasma 03 medical and health sciences 030104 developmental biology Meiosis Arabidopsis Homologous chromosome medicine Arabidopsis thaliana Metaphase 010606 plant biology & botany Fluorescence in situ hybridization |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493998173 |
| Popis: | During meiosis, accurate segregation of chromosomes requires the formation of bivalents at metaphase I. In autopolyploids, there are more than two copies of each chromosome with the same chance to form chiasmata at meiosis. This leads to the formation of multivalent configurations in which chiasma quantification is rather complicated. Here, we present an improved cytological protocol, including fluorescence in situ hybridization, to obtain high quality spreads of metaphase I chromosomes from Arabidopsis thaliana autotetraploids. This method allows an accurate analysis of the different meiotic configurations and enables the assessment of the number of chiasmata formed by each tetrasome (group of four homologs). |
| Databáze: | OpenAIRE |
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