Insecticide pyriproxyfen (Dragón®) damage biotransformation, thyroid hormones, heart rate, and swimming performance of Odontophrynus americanus tadpoles
| Autor: | Candela Soledad Martinuzzi, Agustín Bassó, Carlina Colussi, Andres Maximiliano Attademo, Paola M. Peltzer, Rafael Carlos Lajmanovich |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Environmental Engineering Aché Health Toxicology and Mutagenesis 0208 environmental biotechnology AMPHIBIANS 02 engineering and technology 010501 environmental sciences 01 natural sciences Ciencias Biológicas Carboxylesterase chemistry.chemical_compound THYROID SWIMMING TRACK Internal medicine medicine Environmental Chemistry 0105 earth and related environmental sciences Triiodothyronine ENZYMATIC BIOMARKERS biology Chemistry PYRIPROXYFEN Public Health Environmental and Occupational Health HEART BEATING General Medicine General Chemistry Glutathione biology.organism_classification Pollution Acetylcholinesterase language.human_language 020801 environmental engineering Endocrinology Toxicity language Otros Tópicos Biológicos Pyriproxyfen Odontophrynus americanus CIENCIAS NATURALES Y EXACTAS |
| Popis: | Odontoprynus americanus tadpoles were used to determine the safety concentration of pyriproxyfen (PPF) insecticide by acute and sublethal toxicity tests (nominal range tested 0.01 to 10 [± 15%] PPF mg/L). Median lethal concentration (LC50) and no, and lowest-observed-effect concentrations (NOEC and LOEC, respectively) were calculated. We also assessed the effect on the activities of glutathione S-transferse (GST), acetylcholinesterase (AChE), and carboxylesterase (CbE) and compared to control (CO) tadpoles. Based on the 48-h NOEC value, two sublethal concentrations of PPF (0.01 and 0.1 mg/L) were assayed to detect effects on enzymes activities (GST and CbE), thyroid hormone´s levels (triiodothyronine; T3 and thyroxine; T4), heart function, and tadpoles swimming behaviour. The results showed that the LC50 values of O. americanus tadpoles were 3.73 PPF mg/L and 2.51 PPF mg/L at 24-h and 48-h, respectively (NOEC = 0.1 mg/L; LOEC = 1 mg/L, for both times). PPF concentrations at 48 h, induced enzymatic activities such as GST (212.98%?242.94%), AChE (142.15%?165.08%), and CbE (141.86%?87.14%) significantly respect to COs. During the 22 days of chronic PPF exposure, GST (0.01 mg/L 88%?153% NOEC), AChE (177.82% NOEC), and T4 (70% NOEC) also significantly increased respect to COs. Similarly, heart rate (fH) and ventricular cycle length (VV interval) in CO tadpoles were significantly higher than PPF treated. Finally, at NOEC tadpoles exhibited significant effects on the behavioral endpoint (swimming distance, mean speed, and global activity; P < 0.05). Fil: Lajmanovich, Rafael Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina Fil: Peltzer, Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinuzzi, Candela Soledad. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Basso, Agustin. Universidad Nacional del Litoral; Argentina Fil: Colussi, Carlina Leila. Universidad Nacional del Litoral; Argentina |
| Databáze: | OpenAIRE |
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