Androgen Receptor Regulates Expression of Skeletal Muscle–Specific Proteins and Muscle Cell Types
| Autor: | Huei-Ju Ting, Saleh Altuwaijri, Meng-Yin Tsai, Shuyuan Yeh, Chawnshang Chang, Qingquan Xu, Hong-Chiang Chang, LeRoy A Hanchett, Kuang-Hsiang Chuang, Zhiming Yang, Dong Kun Lee |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Cellular differentiation Muscle Fibers Skeletal Down-Regulation Muscle Proteins Mice Inbred Strains Cell Line Myoblasts Mice Endocrinology Skeletal muscle cell differentiation Internal medicine medicine Animals Myocyte Muscle Skeletal Insulin-like growth factor 1 receptor Mice Knockout Myogenesis Chemistry Skeletal muscle Cell Differentiation Hindlimb Up-Regulation Muscle Fibers Slow-Twitch medicine.anatomical_structure Receptors Androgen Muscle Fibers Fast-Twitch ITGA7 C2C12 Signal Transduction |
| Zdroj: | Endocrine. 25:27-32 |
| ISSN: | 0969-711X |
| Popis: | C2C12 myoblasts expressing the androgen receptor (AR) were used to analyze the role of androgen-AR signaling pathway in skeletal muscle development. Marked up-regulation of AR expression was observed in differentiated myotubes. A nuclear run-on transcription assay demonstrated that transcription of the AR gene is increased during skeletal muscle cell differentiation. Regulation of skeletal muscle-specific protein expression by the androgen-AR signaling pathway was further analyzed using quadriceps skeletal muscle from wild-type (WT) and AR knock-out (ARKO) male mice. A histological analysis of quadriceps skeletal muscle indicates no morphological differences between ARKO and WT mice. However, the androgen-AR signaling pathway increases expression of slow-twitch-specific skeletal muscle proteins and downregulates fast-twitch-specific skeletal muscle proteins, resulting in an increase of slow-twitch muscle fiber type cells in quadriceps muscle. |
| Databáze: | OpenAIRE |
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