Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters
| Autor: | Massoud Amanlou, F. Khosravi, Mojtaba Rezazadeh Valojerdi, L. Karimian, Farid Abolhassani |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system Urology Semen DNA Fragmentation Antioxidants Male infertility Andrology chemistry.chemical_compound Endocrinology medicine Humans Reactive nitrogen species Infertility Male chemistry.chemical_classification Reactive oxygen species urogenital system Superoxide General Medicine medicine.disease Sperm Reactive Nitrogen Species Spermatozoa chemistry DNA fragmentation Reactive Oxygen Species Peroxynitrite |
| Zdroj: | AndrologiaReferences. 46(1) |
| ISSN: | 1439-0272 |
| Popis: | The objective of this study was to investigate the association between the amount of superoxide anion, peroxynitrite as oxidative stress (OS) markers and total antioxidant capacity (TAC) with sperm DNA fragmentation in infertile men with abnormal semen parameters. Semen samples were obtained from 102 infertile couples and divided into groups with normal and abnormal semen parameters according to the World Health Organization (WHO). Peroxynitrite and superoxide anions were detected using spectrofluorometric assays combined with 2,7 dicholorofluorescein (DCF)-DA and 4-chloro-7-nitrobenzo-2-oxa -1, 3-diazole (NBD-CL). Colorimetric assay was used for evaluation of TAC, while DNA fragmentation was studied by using sperm chromatin dispersion test. Superoxide anion, peroxynitrite and DNA fragmentation were significantly higher in infertile couples with abnormal semen parameters as compared to infertile couples with normal semen (P < 0.01). TAC was significantly lower in infertile men with abnormal semen parameters (P < 0.01). There was also a significant positive correlation between OS markers with sperm DNA fragmentation (r = 0.59, P < 0.01 and r = 0.67, P < 0.01, respectively). We have found that imbalance between superoxide anion and peroxynitrite with antioxidant capacity in infertile men with abnormal sperm parameters is associated with higher sperm DNA fragmentation. |
| Databáze: | OpenAIRE |
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