Dynamic pH junction technique for on-line preconcentration of peptides in capillary electrophoresis
| Autor: | Jong-Bok Kim, Shigeru Terabe, Koshi Imami, Maria Rowena N. Monton, Machiko Nakanishi |
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| Rok vydání: | 2005 |
| Předmět: |
Analyte
Chromatography Resolution (mass spectrometry) Chemistry Isoelectric focusing Capillary action Angiotensin II Organic Chemistry Detector Analytical chemistry Electrophoresis Capillary General Medicine Buffers Hydrogen-Ion Concentration Biochemistry Analytical Chemistry law.invention Electrophoresis Capillary electrophoresis law Flow Injection Analysis Angiotensin I Spark plug Peptides |
| Zdroj: | Journal of chromatography. A. 1079(1-2) |
| ISSN: | 0021-9673 |
| Popis: | A method based on the presence of a dynamic pH junction within the capillary to induce band narrowing for enhanced detection sensitivity for some peptides is presented. This technique is predicated on a sharp reduction in an analyte's migration velocity following a reversal of its electrophoretic direction from the acidic sample zone to the basic BGS zone. Larger-than-usual injection volumes of samples in relatively high-conductivity matrices were enabled, without degrading peak shape, resolution and efficiency. The size of the original sample plug was reduced by as much as 38-fold, and improvement in detector response in terms of peak height by as much as 124-fold was obtained. The effects of pH and concentration of the sample matrix, and the length of sample injection on the efficiency of the technique are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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