Genetic variation of varicella-zoster virus strains circulating in Mexico City
| Autor: | Alejandro Escobar-Gutiérrez, Elizabeth González-Durán, José Carmen Gudiño-Rosales, José Ernesto Ramírez-González, Gilberto Vaughan, Araceli Rodríguez-Castillo |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Herpesvirus 3 Human Adolescent Genotype viruses Biology medicine.disease_cause Herpes Zoster Young Adult Virology Genetic variation medicine Humans Child Mexico Genotyping Genetics Molecular Epidemiology Genetic diversity Molecular epidemiology Varicella zoster virus Genetic Variation Infant Middle Aged Cross-Sectional Studies Infectious Diseases Child Preschool Female Viral disease Restriction fragment length polymorphism |
| Zdroj: | Journal of Clinical Virology. 46:349-353 |
| ISSN: | 1386-6532 |
| Popis: | Background Different studies regarding VZV genotype distribution worldwide have demonstrated that genetic diversity and epidemiology of infection significantly vary from region to region. In Mexico, VZV genotype distribution is largely unknown mostly due to the lack of a surveillance system that monitors accurately the presence of viral strains circulating in the country. Objective To identify the main VZV genotypes circulating in the metropolitan area of Mexico City. Study design In this study, 127 different VZV isolates, obtained from residents of the Mexico City Metropolitan area from 2005 to 2008, were identified and genotyped. Viral detection and preliminary genotyping was performed by amplification of the VZV ORF-38 and -54 and RFLP analysis using PstI and BglI endonuclease restriction patterns, respectively. Genotype was confirmed by nucleotide sequence variation along the ORF-22. Results RFLP analysis classified 121 viral strains as European and 6 as mosaic genotype. Genotyping scheme based on the ORF-22 sequence variation identified 120 viral strains belonging to the E genotype, 6 M1 and 1 M4 genotype strains. Conclusions VZV European genotype appears to predominate in Mexico City. This is the first study addressing VZV genotype distribution in Mexico. The information reported in this paper may be useful for future epidemiological studies conducted in the country and also contributes to understand better the molecular epidemiology of VZV in the Americas. |
| Databáze: | OpenAIRE |
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