Overexpression of Sal-like protein 4 in head and neck cancer: epigenetic effects and clinical correlations

Autor: Takuya Nakagawa, Satoshi Yamada, Tomoya Kurokawa, Hiroyuki Mineta, Masato Mima, Daiki Mochizuki, John Chadwick Brenner, Kiyoshi Misawa, Atsushi Imai, Shiori Endo, Yuki Misawa, Hideya Kawasaki
Rok vydání: 2020
Předmět:
0301 basic medicine
Adult
Male
Cancer Research
medicine.disease_cause
HNSCC
Disease-Free Survival
Epigenetic regulation
DNA Methyltransferase 3A
Epigenesis
Genetic
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
SALL4
Biomarkers
Tumor
Medicine
Humans
Epigenetics
DNA (Cytosine-5-)-Methyltransferases
Aged
Cell Proliferation
business.industry
Squamous Cell Carcinoma of Head and Neck
Head and neck cancer
Biomarker
General Medicine
Transfection
Methylation
Middle Aged
medicine.disease
Head and neck squamous-cell carcinoma
eye diseases
Up-Regulation
Gene Expression Regulation
Neoplastic
stomatognathic diseases
030104 developmental biology
Oncology
Head and Neck Neoplasms
030220 oncology & carcinogenesis
Cancer research
DNMT3A
Molecular Medicine
Female
business
Carcinogenesis
Transcription Factors
Zdroj: Cellular oncology (Dordrecht). 43(4)
ISSN: 2211-3436
Popis: Background Sal-like protein 4 (SALL4), an embryonic stem cell factor, has been reported to play an essential role in embryogenesis and oncogenesis. As yet, however, the expression and role of this transcription factor in head and neck squamous cell carcinoma (HNSCC) has not been established.Methods We assessed SALL4 mRNA expression in a well-characterised dataset of 230 HNSCC samples (test cohort 110 cases and validation cohort 120 cases). We also transfected HNSCC cells (FaDu and UM-SCC-6) with SALL4 siRNA and assessed its effects on proliferation and expression of specific epigenetic factors in order to uncover the role of SALL4 in HNSCC.Results Overexpression of SALL4 was detected in tumour samples of both cohorts. HNSCC cells treated with SALL4 siRNA showed a reduction in growth and a decrease in DNA methyltransferase 3 alpha (DNMT3A) expression. In the patient cohorts, SALL4 overexpression was found to significantly correlate with disease recurrence (p
Databáze: OpenAIRE
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