Lipoprotein Lipase Gene Expression in Rat Adipocytes Is Regulated by Isoproterenol and Insulin through Different Mechanisms
| Autor: | Arthur Gutierrez-Hartmann, William M. Wood, Daniel C. Rule, David F. Gordon, Robert H. Eckel, Mary V. Raynolds, Paul D. Awald |
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| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty Transcription Genetic medicine.medical_treatment Molecular Sequence Data Biology chemistry.chemical_compound Endocrinology Transcription (biology) Sequence Homology Nucleic Acid Internal medicine Adipocyte Gene expression medicine Animals Insulin Amino Acid Sequence RNA Messenger Northern blot Cloning Molecular Molecular Biology Messenger RNA Lipoprotein lipase Base Sequence digestive oral and skin physiology Isoproterenol Nucleic Acid Hybridization nutritional and metabolic diseases Rats Inbred Strains General Medicine Enzyme assay Rats Lipoprotein Lipase Adipose Tissue Gene Expression Regulation chemistry biology.protein lipids (amino acids peptides and proteins) DNA Probes |
| Zdroj: | Molecular Endocrinology. 4:1416-1422 |
| ISSN: | 1944-9917 0888-8809 |
| DOI: | 10.1210/mend-4-9-1416 |
| Popis: | Lipoprotein lipase (LPL) is highly regulated by catecholamines and insulin in adipocytes. Isoproterenol, a beta-adrenergic agonist, decreases LPL enzyme activity, whereas insulin increases LPL activity. We have isolated an 868-basepair rat LPL cDNA clone to assess hormone-mediated changes in LPL steady state mRNA levels and LPL gene transcription rates in adipocytes. Northern blot analysis of isoproterenol-treated (10(-6) M) adipocytes showed that LPL steady state mRNA decreased by 15 min. Nuclear run-on transcription assays in isoproterenol-treated cells indicated that LPL gene transcription was also decreased at 15 min compared to that in control cells. Conversely, insulin (6.7 x 10(-8) M) mediated an increase in LPL steady state mRNA in treated adipocytes, yet LPL gene transcription was not different from that in control cells. Thus, the isoproterenol-mediated decrease in LPL enzyme activity and steady state mRNA levels in adipocytes is associated with decreases in LPL gene transcription. Insulin, which does not affect LPL gene transcription, increases LPL enzyme activity and steady state mRNA levels. The effect of insulin on LPL mRNA is probably due to insulin-induced changes in mRNA stability. |
| Databáze: | OpenAIRE |
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