Jeotgalicoccus schoeneichii sp. nov. isolated from exhaust air of a pig barn
Autor: | Tanita Kleinhagauer, Kerstin Klug, Peter Kämpfer, Stefanie P. Glaeser, Udo Jäckel |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
DNA Bacterial Sequence analysis Swine Sus scrofa Spermine Biology medicine.disease_cause Microbiology Staphylococcaceae Agar plate 03 medical and health sciences chemistry.chemical_compound Germany RNA Ribosomal 16S medicine Polyamines Animals Ecology Evolution Behavior and Systematics Phylogeny chemistry.chemical_classification Base Composition Streptococcus Fatty Acids Fatty acid Nucleic Acid Hybridization Vitamin K 2 General Medicine Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA Housing Animal Bacterial Typing Techniques Spermidine 030104 developmental biology chemistry |
Zdroj: | International journal of systematic and evolutionary microbiology. 66(9) |
ISSN: | 1466-5034 |
Popis: | A Gram-staining-positive, non-motile, non-spore-forming, coccus (strain 140805-STR-02T) was isolated from exhaust air of a pig barn on Columbia Blood Agar Base (Oxoid) supplemented with 5 % defibrinated horse blood, Streptococcus selective supplement and 0.5 mg erythromycin l−1. The strains shared high 16S rRNA gene sequence similarity to Jeotgalicoccus pinnipedialis (98.6 %) but only a maximum of 94 % sequence similarity to all other species of the genus Jeotgalicoccus . DNA–DNA hybridisation values between strain 140805-STR-02T and J. pinnipedialis CIP 107946T were 60.3 % (reciprocal, 51.2 %). The quinone system of 140805-STR-02T contained predominantly menaquinone MK-7 and minor amounts of MK-6. The polar lipid profile of strain 140805-STR-02T contained the major compounds diphosphatidylglycerol and phosphatidylglycerol and four unidentified lipids present in minor to moderate amounts. In the polyamine pattern spermidine and spermine were predominant. The fatty acid profile comprising iso-C15 : 0 and anteiso-C15 : 0 as major fatty acids, and was in congruence with those reported for other species of the genus Jeotgalicoccus and thus supported the affiliation of strain 140805-STR-02T to this genus. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain 140805-STR-02T from the most closely related species. Strain 140805-STR-02T represents a novel species, for which the names Jeotgalicoccus schoeneichii sp. nov. is proposed, with the type strain 140805-STR-02T (=LMG 29445T=CCM 8667T). |
Databáze: | OpenAIRE |
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