LNCIB human full-length cDNAs collection: towards a better comprehension of the human transcriptome
| Autor: | Enio Klaric, Luigi Marchionni, Danilo Licastro, Dejan Lazarevic, Nabil Bahar, Giacomo Marcuzzi, James F. Reid, Riccardo Marzio, Emiliano Dalla, Claudio Schneider, Roberto Verardo, Anna Belgrano |
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| Rok vydání: | 2004 |
| Předmět: |
Genetics
General Immunology and Microbiology Databases Factual Transcription Genetic cDNA library Genome Human UniGene General Medicine Biology General Biochemistry Genetics and Molecular Biology Transcriptome Complementary DNA RefSeq Humans Genomic library Human genome General Agricultural and Biological Sciences Functional genomics Gene Library |
| Zdroj: | Comptes rendus biologies. 326(10-11) |
| ISSN: | 1631-0691 |
| Popis: | LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes. |
| Databáze: | OpenAIRE |
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