Static and magic angle spinning (31)P NMR spectroscopy of two natural plasma membranes
| Autor: | J. Segalen, E. Le Rumeur, M. Le Floc'h, Laurent Metzinger, Geneviève Leray, Céline Moreau, J. D. De Certaines |
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| Rok vydání: | 1999 |
| Předmět: |
Magnetic Resonance Spectroscopy
Biophysics Phospholipid Analytical chemistry Biochemistry Spectral line chemistry.chemical_compound Membrane Lipids Sarcolemma Phospholipid composition Structural Biology Phosphatidylcholine Genetics Magic angle spinning Animals Humans Molecular Biology Phospholipids Chemistry Bilayer arrangement Bilayer Cell Membrane Erythrocyte Membrane Phosphorus Isotopes Cell Biology Plasma Crystallography Membrane Phosphatidylcholines lipids (amino acids peptides and proteins) 31p nmr spectroscopy 31P NMR spectroscopy Rabbits Plasma membrane |
| Zdroj: | FEBS letters. 461(3) |
| ISSN: | 0014-5793 |
| Popis: | Static and magic angle spinning 31P NMR spectroscopy was used for the first time in natural plasma membranes from erythrocytes and skeletal muscle to study phospholipid arrangement and composition. Typical static powder-like spectra were obtained showing that phospholipids were in a bilayer arrangement. Magic angle spinning narrowed spectra into two components. The first one corresponded to phosphatidylcholine and the second one to the other phospholipids with intensities in agreement with the known phospholipid composition. These findings show that NMR data previously acquired using model membranes can be transposed to studies on phospholipids in their natural environment. |
| Databáze: | OpenAIRE |
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