Evaluation of Toxicity in Mouse Bone Marrow Progenitor Cells
| Autor: | Sebastian Medina, Peace C. Ezeh, Huan Xu, Scott W. Burchiel, Shu Chun Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Myeloid Population Biology Toxicology Article Xenobiotics Colony-Forming Units Assay 03 medical and health sciences Mice 0302 clinical medicine Bone Marrow medicine Animals Progenitor cell education education.field_of_study Stem Cells Flow Cytometry Hematopoietic Process Hematopoiesis Haematopoiesis 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Toxicity Cancer research Environmental Pollutants Bone marrow Stem cell |
| Zdroj: | Current Protocols in Toxicology |
| ISSN: | 1934-9262 |
| Popis: | Development of blood cells through hematopoiesis occurs in the bone marrow (BM), and can be adversely impacted by various substances and/or conditions ranging from known therapeutic, intentionally administered xenobiotics to unintentional food additives and exposure to environmental chemicals. The principles underlying the techniques for evaluating toxicity to BM progenitors (erythroid, myeloid, and lymphoid) exploit changes in the normal hematopoietic process, biochemical cell surface and intracellular markers, as well as components of the BM microenvironment. Toxicological investigations following in vivo exposures of mice or in vitro exposures of mouse primary BM cell cultures allow the assessment of the developmental and functional integrity of BM cells, cell population shifts, and adverse biochemical effects due to toxicity. Colony forming unit (CFU) assays and flow cytometry are indispensable techniques in these toxicity studies. |
| Databáze: | OpenAIRE |
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