Polyribosomes in protoplasts isolated from tobacco leaves

Autor:	Mettrie R, Farkas Gl, P. Ruzicska, G. Premecz, Yuri L. Dorokhov, T. Oláh
Rok vydání:	1979
Předmět:	Osmotic shock biology fungi food and beverages Plant Science biochemical phenomena metabolism and nutrition Protoplast biology.organism_classification Ribosome Molecular biology Plasmolysis Biochemistry Callus Polysome Genetics Protein biosynthesis bacteria Nicotiana
Zdroj:	Planta. 145:199-203
ISSN:	1432-2048 0032-0935
DOI:	10.1007/bf00388718
Popis:	Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a44f7f34e7e55fccae56d08a7bf90bdc https://doi.org/10.1007/bf00388718 Zobrazit plný text záznamu Full text from SpringerLink