Characterization of inflammatory reaction induced by neuwiedase, a P-I metalloproteinase isolated from Bothrops neuwiedi venom
| Autor: | Carolina de Freitas Oliveira, Maria Inês Homsi-Brandeburgo, Jaqueline das Dores Dias Oliveira, Veridiana M. Rodrigues, Luiz Ricardo Gourlart, Amélia Hamaguchi, Tânia M. Alcântara, Daiana Silva Lopes, Cristiani Baldo, Patricia Bianca Clissa, Ana Maria Moura-da-Silva |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Chemokine
Necrosis medicine.medical_treatment Inflammation Viper Venoms Biology Pharmacology Toxicology Cell Line Mice medicine Animals Myocyte Bothrops Muscle Skeletal Mice Inbred BALB C Reverse Transcriptase Polymerase Chain Reaction Metalloendopeptidases Skeletal muscle biology.organism_classification Cytokine medicine.anatomical_structure Snake venom Immunology biology.protein Cytokines Chemokines medicine.symptom |
| Zdroj: | Toxicon. 54:42-49 |
| ISSN: | 0041-0101 |
| Popis: | The Snake Venom Metalloproteinases (SVMPs) play a relevant role in the multifactorial inflammatory response induced by Bothrops envenomations. Neuwiedase, an SVMP isolated from Bothrops neuwiedi venom, is devoid of hemorrhagic activity on skin tests, but is able to induce myonecrosis and degrade fibrinogen, fibrin, type I collagen, fibronectin and laminin. In this study, we analyzed the inflammatory reaction induced by neuwiedase in gastrocnemius muscle, with special focus on cytokines release. Our results showed clear evidence of inflammatory infiltrate in the gastrocnemius muscle and an increase of MMP-9, and the cytokines KC, IL-1 beta and IL-6 in the early periods after toxin injection. The cytokine release was also evaluated in inflammatory and muscular cell culture. Both murine peritoneal adherent cells (MPACs) and muscle cells (C2C12) released pro-inflammatory cytokines after stimulus with neuwiedase. MPACs showed increased production of KC, IL-1 beta and IL-6 in the cell culture supernatant while in C2C12, the predominant chemokine expressed was KC. These data reinforce the importance of SVMPs in the inflammatory response caused by envenomation and point out the role of muscle cells in this event by releasing pro-inflammatory mediators able to attract leukocytes to the muscle, thus starting and amplifying the setting of the inflammatory reaction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect