Transportation stress in young bulls alters expression of neutrophil genes important for the regulation of apoptosis, tissue remodeling, margination, and anti-bacterial function
| Autor: | Mark A. Crowe, Bernadette Earley, K.R. Buckham Sporer, Jeanne L. Burton |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Neutrophils Immunology Inflammation Apoptosis Transportation Biology Animal Welfare Immune system Stress Physiological Internal medicine Gene expression medicine Animals Respiratory system Animal Husbandry Regulation of gene expression General Veterinary Bacteria Fas receptor Endocrinology Gene Expression Regulation Cattle medicine.symptom Wound healing |
| Zdroj: | Veterinary immunology and immunopathology. 118(1-2) |
| ISSN: | 0165-2427 |
| Popis: | The transportation of beef cattle results in a stress response that is associated with increased susceptibility and severity of respiratory diseases, presumably due to an alteration in immune function. Neutrophils are phagocytic immune cells important in lung defense and are also targets of the stress response. The objective of this study was to determine if a 9h transportation of young bulls by road induced changes in the expression of candidate genes known to be important in neutrophil-mediated defense and inflammation in the lung. These neutrophil genes encompassed functions of apoptosis (A1 and Fas), tissue remodeling (MMP-9), vascular margination (L-selectin), bacterial killing (BPI), and wound healing (betaglycan), as well as responsiveness of the cells to stress-induced increases in glucocorticoid hormones (GRalpha). To explore gene expression changes, blood was collected, plasma harvested, and neutrophils isolated from six Belgian Blue x Friesian bulls (231+/-7.0 kg in weight; 282+/-4 days of age) at -24, 0, 4.5, 9.75, 14.25, 24, and 48h relative to commencement of a 9h road transportation by truck. Plasma cortisol concentrations were elevated at 4.5 and 9.75h, peaking at 50.64+/-4.46 ng/mL (P |
| Databáze: | OpenAIRE |
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