Contractile effects of endothelins on isolated ampullar segment of human oviduct in luteal phase of menstrual cycle
| Autor: | Snezana V. Jankovic, Slobodan M. Jankovic, Srdjan Stefanovic, Snezana Cupara, Vesela Radonjic, Goran Lukic |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
medicine.hormone medicine.medical_specialty media_common.quotation_subject In Vitro Techniques Luteal Phase Luteal phase Biology Peptides Cyclic Endothelins chemistry.chemical_compound Piperidines Internal medicine Follicular phase medicine Humans Receptor Fallopian Tubes Menstrual cycle media_common Pharmacology Dose-Response Relationship Drug Endothelin-1 BQ-788 Middle Aged Endocrinology chemistry Oviduct Female medicine.symptom Oligopeptides Muscle Contraction Muscle contraction |
| Zdroj: | Pharmacological Research. 59:69-73 |
| ISSN: | 1043-6618 |
| Popis: | Endothelin-1 induces contractions of human oviduct ampullar segment in follicular phase of menstrual cycle, acting on ET(A) receptors. The aim of our study was to investigate effects of endothelin-1, endothelin-2 and endothelin-3 on isolated ampullar segment of human oviducts, taken from the patients in luteal phase of menstrual cycle. Fallopian tubes were taken from 20 female patients (one tube from each patient) during abdominal hysterectomy with adnexectomy, due to extensive uterine fibroids. The oviduct ampulla was mounted in an organ bath longitudinally, and the tension of the isolated preparation was recorded with the isometric transducer. Endothelin-1 produced concentration-dependent tonic contraction of the isolated ampullar segment (EC(50)=6.80 +/- 1.2 x 10(-10)M), and concentration-dependent inhibition of its rhythmic contractions (EC(50)=7.86 +/- 2.3 x 10(-10)M). Endothelin-2 produced concentration-dependent tonic contraction of the isolated ampullar segment (EC(50)=4.56 +/- 0.3 x 10(-10)M), without affecting its rhythmic contractions. Endothelin-3 did not affect either tone or rhythmic contractions of the isolated preparations. Selective antagonist for ET(A) receptor subtype, BQ 123, produced inhibition of endothelin-1 effects on both tone (pA(2)=9.50) and spontaneous rhythmic contractions (pA(2)=10.73), while selective antagonist for ET(B) receptor subtype, BQ 788, produced only inhibition of endothelin-1 effects on tone (pA(2)=9.61), while the effect of endothelin-1 on spontaneous rhythmic contractions remained unaffected. The results of our study suggest that in the luteal phase both ET(A) and ET(B) receptors regulate tone, and only ET(A) receptors regulate rhythmic activity of human oviduct's ampullar segment. |
| Databáze: | OpenAIRE |
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