Detection of Porcine Epidemic Diarrhea Virus Using Polymerase Chain Reaction and Comparison of the Nucleocapsid Protein Genes among Strains of the Virus
| Autor: | Shuichi Kubota, Katsuhiko Amimoto, Osamu Sasaki, Hisao Yasuhara, Nobutaka Okada, Takashi Kitazima |
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| Rok vydání: | 1999 |
| Předmět: |
Diarrhea
Swine Molecular Sequence Data Sequence alignment Biology Polymerase Chain Reaction Virus law.invention law Sequence Homology Nucleic Acid Animals Nucleocapsid Gene Polymerase chain reaction Swine Diseases Base Sequence General Veterinary Strain (chemistry) biology.organism_classification Virology Coronavirus Nucleic acid Coronavirus Infections Porcine epidemic diarrhea virus Sequence Alignment Nested polymerase chain reaction |
| Zdroj: | Journal of Veterinary Medical Science. 61:827-830 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.61.827 |
| Popis: | Two pairs of PCR primers were designed to perform nested PCR targetting of a 540 bp fragment of the nucleocapsid (N) protein gene (N gene) of porcine epidemic diarrhea virus (PEDV). The N gene of PEDV was amplified with 4 PEDV strains and 11 small intestines of PEDV-infected piglets collected from 2 farms in Kagoshima prefecture, Japan. Nucleotide sequences of the PCR products from a Korean and two Japanese strains (KKN96-1 and S1) of PEDV isolated in 1993 and 1996, respectively, were almost identical. These results suggest that the PCR is an available tool for detection of PEDV from pigs in the field, and that the two Japanese strains (KKN96-1 and S1) were genetically similar to the Korean strain. |
| Databáze: | OpenAIRE |
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