The carboxy-terminal extension of the D1-precursor protein is dispensable for a functional photosystem II complex in Chlamydomonas reinhardtii
| Autor: | Silke Schrader, Udo Johanningmeier |
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| Rok vydání: | 1992 |
| Předmět: |
Photosystem II
Protein subunit Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins Restriction Mapping Mutant Chlamydomonas reinhardtii Plant Science Biology medicine.disease_cause Genetics medicine Animals Amino Acid Sequence Cloning Molecular Codon Gene Mutation Base Sequence Mutagenesis Photosystem II Protein Complex Exons General Medicine biology.organism_classification Stop codon Oligodeoxyribonucleotides Biochemistry Mutagenesis Site-Directed Chromosome Deletion Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 19:251-256 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/bf00027346 |
| Popis: | The D1-precursor protein of the photosystem II reaction centre contains a carboxy-terminal extension whose proteolytic removal is necessary for oxygen-evolving activity. To address the question of the role of the carboxy-terminal extension in the green alga Chlamydomonas reinhardtii, we truncated D1 by converting codon Ser345 of the psbA gene into a stop codon. Particle gun transformation of an in vitro modified psbA gene fragment also carrying mutations conferring herbicide resistance yielded a homoplasmic transformant containing the stop codon. Since oxygen evolution capacity is not affected in this mutant as compared with herbicide-resistant control cells, the carboxy-terminal extension is dispensable for a functional photosystem II complex under normal growth conditions. |
| Databáze: | OpenAIRE |
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