Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana
| Autor: | Luis Lopez-Molina, Urszula Piskurewicz |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Germination/drug effects 01 natural sciences 03 medical and health sciences chemistry.chemical_compound food Endosperm/drug effects/metabolism Arabidopsis Botany Radicle Arabidopsis/drug effects/metabolism Abscisic acid biology Abiotic stress fungi food and beverages Abscisic Acid/pharmacology Gene Expression Regulation Plant/drug effects Gibberellins/pharmacology Seeds/drug effects/metabolism biology.organism_classification ddc:580 030104 developmental biology chemistry Germination Seedling Arabidopsis Proteins/metabolism Gibberellin Cotyledon 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493933549 Environmental Responses in Plants : Methods and protocols |
| ISSN: | 1940-6029 |
| DOI: | 10.1007/978-1-4939-3356-3_15 |
| Popis: | The model organism Arabidopsis thaliana has been extensively used to unmask the molecular genetic signaling pathways controlling seed germination in plants. In Arabidopsis, the normal seed to seedling developmental transition involves testa rupture soon followed by endosperm rupture, radicle elongation, root hair formation, cotyledon expansion, and greening. Here we detail a number of basic procedures to assess Arabidopsis seed germination in response to different light (red and far-red pulses), temperature (seed thermoinhibition), and water potential (osmotic stress) environmental conditions. We also discuss the role of the endosperm and how its germination-repressive activity can be monitored genetically by means of a seed coat bedding assay. Finally we detail how to evaluate germination responses to changes in gibberellin (GA) and abscisic acid (ABA) levels by manipulating pharmacologically the germination medium. |
| Databáze: | OpenAIRE |
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