INCREASED RELEASE OF INTERLEUKIN 6 BY ESOPHAGEAL MUCOSA IN CHILDREN WITH REFLUX ESOPHAGITIS
| Autor: | Giuseppe M. Pontieri, P. Rea, C. Pacchiarotti, Ettore Cardi, Alessandra Zicari, Fabio Cerroni, Giovanni Corrado, M. Cavaliere |
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| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Adolescent Biopsy Enzyme-Linked Immunosorbent Assay Fibrinogen Gastroenterology Endoscopy Gastrointestinal Esophagus Internal medicine medicine Humans Prospective Studies Interleukin 6 Prospective cohort study Child Esophagitis Peptic Cells Cultured Monitoring Physiologic Mucous Membrane Hepatology biology medicine.diagnostic_test business.industry Esophageal disease Interleukin-6 Hydrogen-Ion Concentration medicine.disease medicine.anatomical_structure C-Reactive Protein biology.protein Female Complication business Esophagitis medicine.drug |
| Popis: | Objective To evaluate the release of interleukin-6 (IL-6) by oesophageal mucosa and to establish the serum levels of IL-6 and C-reactive protein (CRP), and plasma fibrinogen in children with reflux oesophagitis. Design In a prospective study, IL-6 release by tissue fragments obtained from oesophageal biopsies was determined and serum IL-6 and CRP as well as plasma fibrinogen were analysed. Methods The study population comprised ten children with reflux oesophagitis, diagnosed on the basis of 24 h oesophageal pH monitoring and endoscopy with biopsies. Ten children with recurrent abdominal pain were studied for comparative purposes. Biopsy tissue fragments were processed to obtain a cell suspension and the release of IL-6 was determined in culture medium. Serum IL-6 levels were measured by ELISA, serum CRP by turbidimetry, and plasma fibrinogen by spectrophotometry. Results Oesophageal cells obtained from reflux oesophagitis patients synthesize and release in vitro a significantly higher amount of IL-6 than controls (71.26 ± 19.5 versus 31.67 ± 8.02 pg/10 6 cells; P< 0.01). Serum IL-6, serum CRP and plasma fibrinogen levels were not statistically different between patients with reflux oesophagitis and controls. Conclusions These results suggest a short-term action of IL-6 since its effects could be exerted only in the microenvironment of the oesophageal mucosa. |
| Databáze: | OpenAIRE |
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