A Hyaluronan and Platelet-Rich Plasma Hydrogel for Mesenchymal Stem Cell Delivery in the Intervertebral Disc: An Organ Culture Study
| Autor: | Luca Ambrosio, Jan Gewiess, Sebastian Wangler, Marianna Peroglio, Rocco Papalia, Vincenzo Denaro, Mauro Alini, Gianluca Vadalà, Fabrizio Russo, Sibylle Grad, Wei Guo |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Intervertebral Disc Degeneration Matrix (biology) lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine Hyaluronic acid hyaluronic acid 610 Medicine & health lcsh:QH301-705.5 Spectroscopy Cell Differentiation Hydrogels General Medicine musculoskeletal system Computer Science Applications Cell biology medicine.anatomical_structure endocrine system Nucleus Pulposus Mesenchymal Stem Cell Transplantation Organ culture complex mixtures Article Catalysis Inorganic Chemistry 03 medical and health sciences organ culture Organ Culture Techniques medicine Animals Humans Viability assay Physical and Theoretical Chemistry Molecular Biology Keratin-19 030203 arthritis & rheumatology mesenchymal stem cells Regeneration (biology) Organic Chemistry Mesenchymal stem cell Batroxobin technology industry and agriculture platelet-rich plasma Intervertebral disc equipment and supplies 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation chemistry Platelet-rich plasma ex vivo Cattle intervertebral disc |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 6 International Journal of Molecular Sciences, Vol 22, Iss 2963, p 2963 (2021) Russo, Fabrizio; Ambrosio, Luca; Peroglio, Marianna; Guo, Wei; Wangler, Sebastian; Gewiess, Jan; Grad, Sibylle; Alini, Mauro; Papalia, Rocco; Vadalà, Gianluca; Denaro, Vincenzo (2021). A Hyaluronan and Platelet-Rich Plasma Hydrogel for Mesenchymal Stem Cell Delivery in the Intervertebral Disc: An Organ Culture Study. International journal of molecular sciences, 22(6) MDPI 10.3390/ijms22062963 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22062963 |
| Popis: | The purpose of the present pilot study was to evaluate the effect of a hydrogel composed of hyaluronic acid (HA) and platelet-rich plasma (PRP) as a carrier for human mesenchymal stem cells (hMSCs) for intervertebral disc (IVD) regeneration using a disc organ culture model. HA was mixed with batroxobin (BTX) and PRP to form a hydrogel encapsulating 1 × 106 or 2 × 106 hMSCs. Bovine IVDs were nucleotomized and filled with hMSCs suspended in ~200 μL of the PRP/HA/BTX hydrogel. IVDs collected at day 0 and nucleotomized IVDs with no hMSCs and/or hydrogel alone were used as controls. hMSCs encapsulated in the hydrogel were also cultured in well plates to evaluate the effect of the IVD environment on hMSCs. After 1 week, tissue structure, scaffold integration, hMSC viability and gene expression of matrix and nucleus pulposus (NP) cell markers were assessed. Histological analysis showed a better preservation of the viability of the IVD tissue adjacent to the gel in the presence of hMSCs (~70%) compared to the hydrogel without hMSCs. Furthermore, disc morphology was maintained, and the hydrogel showed signs of integration with the surrounding tissues. At the gene expression level, the hydrogel loaded with hMSCs preserved the normal metabolism of the tissue. The IVD environment promoted hMSC differentiation towards a NP cell phenotype by increasing cytokeratin-19 (KRT19) gene expression. This study demonstrated that the hydrogel composed of HA/PRP/BTX represents a valid carrier for hMSCs being able to maintain a good cell viability while stimulating cell activity and NP marker expression. |
| Databáze: | OpenAIRE |
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