Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress
| Autor: | Changtao Wang, Wen-Yan Shi, Ming Chen, Wei-Jun Zheng, Ya-Jun Xi, Yong-Bin Zhou, Pan-Pan Lu, Zhao-Shi Xu, Jin-Dong Fu, Jun Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mutant Arabidopsis Plant Science Biology Bax inhibitor-1 Endoplasmic Reticulum 01 natural sciences Heat stress 03 medical and health sciences Downregulation and upregulation Gene Expression Regulation Plant lcsh:Botany Arabidopsis thaliana Protein Interaction Domains and Motifs Gene Triticum Plant Proteins BAX inhibitor 1 Arabidopsis Proteins Endoplasmic reticulum TaFKBP62 Wild type Membrane Proteins food and beverages Intracellular Membranes biology.organism_classification Plants Genetically Modified Cell biology Up-Regulation lcsh:QK1-989 030104 developmental biology Heat-responsive genes Mutation RNA-seq Heat-Shock Response 010606 plant biology & botany Research Article |
| Zdroj: | BMC Plant Biology, Vol 18, Iss 1, Pp 1-12 (2018) BMC Plant Biology |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/s12870-018-1485-0 |
| Popis: | Background Heat stress is a severe environmental stress that affects plant growth and reduces yield. Bax inhibitor-1 (BI-1) is a cytoprotective protein that is involved in the response to biotic and abiotic stresses. The Arabidopsis (Arabidopsis thaliana) BI-1 mutants atbi1–1 and atbi1–2 are hypersensitive to heat stress, and AtBI-1 overexpression rescues thermotolerance deficiency in atbi1 plants. Nevertheless, the mechanism of BI-1 in plant thermotolerance is still unclear. Results We identified a wheat (Triticum aestivum L.) BI-1 gene, TaBI-1.1, which was highly upregulated in an RNA sequencing (RNA-seq) analysis of heat-treated wheat. The upregulation of TaBI-1.1 under heat stress was further demonstrated by real time quantitative PCR (qRT-PCR) and β-glucuronidase (GUS) staining. Compared with the wild type Col-0, the atbi1–2 mutant is hypersensitive to heat stress, and constitutive expression of TaBI-1.1 in atbi1–2 (35S::TaBI-1.1/ atbi1–2) rescued the deficiency of atbi1–2 under heat stress. Furthermore, we identified TaFKBP62 as a TaBI-1.1-interacting protein that co-localized with TaBI-1.1 on the endoplasmic reticulum (ER) membrane and enhanced heat stress tolerance. Additionally, HSFA2, HSFB1, ROF1, HSP17.4B, HSP17.6A, HSP17.8, HSP70B, and HSP90.1 expression levels were suppressed in atbi1–2 plants under heat stress. In contrast, 35S::TaBI-1.1/atbi1–2 relieved the inhibitory effect of AtBI-1 loss of function. Conclusions TaBI-1.1 interacted with TaFKBP62 and co-localized with TaFKBP62 on the ER membrane. Both TaBI-1.1 and AtBI-1 regulated the expression of heat-responsive genes and were conserved in plant thermotolerance. Electronic supplementary material The online version of this article (10.1186/s12870-018-1485-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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